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Claire Riggs, PhD Candidate, Podrabsky Laboratory, Portland State University

Small Noncoding RNA Expression During Extreme Anoxia ToleranceBD

Thursday, May 25, 2017 3:30 PM - 4:30 PM


Small Noncoding RNA Expression During Extreme Anoxia Tolerance

Small noncoding RNAs (sncRNA) have recently emerged as specific and rapid regulators of gene expression, involved in myriad cellular and organismal processes. MicroRNAs, a class of small RNAs, are differentially expressed in diverse taxa in response to environmental stress, including anoxia. Most vertebrates, however, are sensitive to a lack of oxygen, a brief period resulting in severe damage or death. Studies on sncRNA and anoxia have focused on these anoxia-sensitive species. Studying sncRNAs in anoxia-tolerant organisms may provide insight into adaptive mechanisms supporting anoxia tolerance. Embryos of the annual killifish Austrofundulus limnaeus are the most anoxia-tolerant vertebrates known, surviving over 100 days at their peak tolerance. Their anoxia tolerance and physiology vary over development, such that both anoxia-tolerant and anoxia-sensitive organisms comprise the species. This allows for a robust comparison to identify sncRNAs essential to anoxia-tolerance. For this study, RNA sequencing was used to identify and quantify expression of sncRNAs in 4 embryonic stages of A. limnaeus in response to an exposure to anoxia and subsequent aerobic recovery. Unique stage-specific patterns of expression were identified that correlate with anoxia tolerance. In addition, embryos of A. limnaeus appear to constitutively express stress-responsive miRNAs. Most differentially expressed sncRNAs were upregulated during recovery. Many novel groups of sncRNAs with expression profiles suggesting a key role in anoxia tolerance were identified, including small RNAs derived from mitochondrial tRNAs. This global analysis has revealed groups of candidate sncRNAs that we hypothesize are important to anoxia tolerance.  


OSU Hatfield Marine Science Center
Guin Library Seminar Room
2030 SE Marine Science Drive
Newport
OR
Michael Banks
541-867-0420
maryann.bozza at oregonstate.edu
Hatfield Marine Sci Ctr
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